Before doing the breeding of microbes (cultivation) can be isolated in advance to obtain isolates. The definition of isolation is the separation of a specific microbial populations from a naturally occurring mixture. So the result of the isolation process is a species (species) of microbes that are considered essential or are called isolates. To learn more about the microbes we need a population of isolates before and to get this population is the process of cultivation. Thus, isolates obtained in significant amounts. Isolate which is used to multiply the number of microorganisms known as inoculum.
Pure culture is a culture (culture) that contain only one species of microbe. While culture is a culture mixture containing more than one species of microbe. There is also the so-called two mixed cultures (two-membered culture) are two species of microbes that accidentally were grown and maintained in such a way as intertwined with each other.
Generally, laboratory work involving various pure cultures so that if other species of microbes that accidentally get into pure culture, the culture is said to have been contaminated. The possibility of laboratory contamination is enormous, because there are microbes everywhere. For example on the skin and the human hand, dust particles in the air, water and so forth. Therefore to maintain the culture remains pure, then the things that must be considered are:
Pure culture is a culture (culture) that contain only one species of microbe. While culture is a culture mixture containing more than one species of microbe. There is also the so-called two mixed cultures (two-membered culture) are two species of microbes that accidentally were grown and maintained in such a way as intertwined with each other.
Generally, laboratory work involving various pure cultures so that if other species of microbes that accidentally get into pure culture, the culture is said to have been contaminated. The possibility of laboratory contamination is enormous, because there are microbes everywhere. For example on the skin and the human hand, dust particles in the air, water and so forth. Therefore to maintain the culture remains pure, then the things that must be considered are:
- All material to be directly related to the microbe must be sterile.
- All the media used to grow the cells must be sterile
- No contaminants that enter and grow.
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