The process of making chitosan

Preparation of chitosan from shrimp waste as a natural preservative in food consists of two processes namely deproteinization and demineralization. In the process of deproteinization, soaked dried shrimp waste and then blend until smooth then next sifted soaked in 1M NaOH solution with a ratio of 1: 5 for the shell: the solution was stirred in 1 hour and then heated 90 ° C for 1 hour then cooled washed with water until pH neutral and then dried.

As for the second process is by demineralization. Demineralization is done by way of shrimp waste deproteinization results subsequently soaked in a solution of 1M CH3COOH ratio 10: 1 for the solvent: results deproteinasi then stirred 1 hour and then heated 90 ° C for 1 hour after it is cooled and filtered and then washed with water until neutral pH and then dried until a chitin. Then chitin soaked in 1M NaOH solution perb 20: 1 for the solvent: chitin (the results of demineralization phase) and then stirred 1 hour and then heated 140 ° C for 90 minutes then cooled and filtered and then washed with water until pH neutral and then dried so it will be Chitosan. Chitosan which have become dissolved in 15% acetic acid trade then stirred for 5 minutes and apply a Chitosan is on food.